Technology Overview

 

The core technology behind chipcytometry is imaging cytometry. We extended this technology by preserving the cells or tissues in a way that allows to re-stain the samples again and again due to immobilisation of the cells within microfluidic chips. In general, we can analyse any kind of biological cells since they will adhere very efficiently on our microfluidic-chips coated with an innovative cell-adhesive surface modification. After immobilisation (and optional fixation using fixatives like PFA), one can measure a virtually unlimited set of markers or perform functional tests by cyclic staining/imaging/switch-off of remaining fluorescence.

 

Not only that we like serial analysis of markers because of the high number of markers one can analyse. We also like it because you don’t need to establish the markersets before analysis like in flow cytometry. Since all markers can have the same fluorescent dye/label, we can combine them just as we like. So we take our sample and start to explore it…

 

Primarily, this produces a huge number of quite nice looking pictures of fluorescent markers on cells:

The next step is large scale image processing to assign marker expression values to each individual cell. We currently analyse a maximum of about 12000 cells per chip, with the beginning of the beta test-phase in fall 2012 we will analyse about 50000 cells per chip.

 

This produces quite complex data, so we developed data-vizualisation tools that help you to understand the results of your experiment:

The nice thing is that all these data from cluster analysis to raw image are linked, so you can validate your results by following the single data points down to the single cell image.

 

What will be next? We currently work on a robot that will perform all these steps automatically with high speed and high quality.

 



. Chipcytometry is currently sponsored by Go-Bio. Developed at Hannover Medical School, Department of Pediatric Pneumology, Allergology and Neonatology //Disclaimer