We often have been asked if it would be possible to seed cells on our chips and let them grow until confluency and perform assays followed by Chipcytometry on these cells. Well, in theory it should work since the chips are equipped with standard microfluidic connectors. So we gave it a try: We seeded 16 HDE cells on the chips, connected a microfluidic pump to the chip and allowed the medium to recirculate between chip and reservoir for 72h in an incubator.
This image shows the cells after seeding:
After 72h, the cells nicely formed an confluent cell layer:
So let’s use it for some fancy stuff…